Sheep anti cytosine deaminase antibody
货号：2485-4906
规格：1 ml
价格：3120
产品类型：免疫组化一抗
品牌：Bio-Rad
物种：Yeast
宿主：Sheep
抗体亚型：IgG3
荧光染料：Alexa Fluor 488
| 产品介绍 | | |
| Sheep anti cytosine deaminase antibodyrecognizes cytosine deaminase, also known as cytosine aminohydrolase. Cytosine deaminase is a 158 amino acid ~18 kDa enzyme involved in the conversion of cytosine to uracil. Sheep anti Human cytosine deaminase antibody recognizes the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting (Fuerer and Iggo 2004). |
| 产品详情 | | |
| Target Species | Yeast |
| Product Form | Purified IgG - liquid |
| Preparation | Purified Ig prepared by affinity chromatography on Protein G |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunoge | Purified yeast cytosine deaminase. |
| Approx. Protein Concentrations | Ig concentration 5mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/500 | 1/2500 |
| Western Blotting | √ | 1/100 | 1/500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | F,E | 0.5mg |
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F,IF | 0.5mg |
| 文献 |
1.Fuerer, C.et al. (2004) 5-Fluorocytosine increases the toxicity of Wnt-targeting replicating adenoviruses that express cytosine deaminase as a late gene.Gene Ther. 11: 142-512.Hammer, K.et al.(2015) Engineered adenoviruses combine enhanced oncolysis with improved virus production by mesenchymal stromal carrier cells.Int J Cancer. 137 (4): 978-90.3.Amon, A.et al.(2008) Methods and Composition for Diagnosing and Treating CancerUS patent application US 11/767,2004.Pan, X.et al.(2003) Adenovirus-mediated gene transfer in the treatment of pancreatic cancer.Pancreas. 26: 274-8.5.Conrad, C.et al.(2005)Δ24-hyCD adenovirus suppresses glioma growthin vivoby combining oncolysis and chemosensitization.Cancer Gene Ther. 12: 284-94.6.Michnick, S.W.et al.(2010) A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.Methods Enzymol. 470: 335-68.7.Komissarov, A.et al.(2017) Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.Mol Biol Rep. 44 (4): 323-32.8.Hanauer, J.R.H.et al.(2019) High-affinity DARPin allows targeting of measles virus to glioblastoma multiforme in combination with protease targeting without loss of potency.Molecular Therapy - Oncolytics. 14. Oct [Epub ahead of print].
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技术参数 产品应用 Elisa；WB
产品类型 Polyclonal Antibody
推荐用于 Elisa；WB
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