CD45RA Monoclonal Antibody (HI100), Super Bright 702, eBioscience™

货号：67-0458-42

规格：100 Tests

价格：3331

产品类型：流式抗体

品牌：eBioscience

抗原：CD45RA

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HI100

荧光染料：Super Bright 702

抗体类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright 702, eBioscience™
浓度：	5uL/test	用法：	5uL（0.06µg）/test(Flow)

产品详细信息Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.仅用于科研。不用于诊断过程。

数据

CD45RA Antibody (67-0458-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45RO FITC (Product # 11-0457-42) and Mouse IgG2b K Isotype Control Super Bright 702 (Product # 67-4732-82) (left) or Anti-Human CD45RA Super Bright 702 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Scientific reportsIntracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding."67-0458 was used in Flow cytometry/Cell sorting to show microfluidic vortex shedding mediates intracellular delivery of mRNA into primary human T lymphocytes."AuthorsJarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

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