| 产品介绍 | | |
| Mouse anti cortisol monoclonal antibody, clone C13recognizes cortisol, a glucocorticoid hormone synthesized from cholesterol by the adrenal cortex. It is released in response to pituitary adenocorticotrophic hormone (ACTH) which is, in turn, regulated by corticotrophin releasing factor (CRF). High levels of cortisol act to regulate the release of ACTH and CRF via negative feedback inhibition.The release of cortisol stimulates the conversion of proteins to carbohydrates, raises blood sugar levels and promotes glycogen storage in the liver.The following cross reactivities were observed:Cortisol 100%Prednisolone 30%Cortisone 25%Corticosterone 10%11-desoxycortisol 5%No reactivity was observed with 17-a-OH-progesterone. |
| 产品详情 | | |
Target Species | Broad |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | Cortisol conjugated to bovine serum albumin. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/50 | 1/1500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E;WB | 0.5 mg |
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F;IF | 0.1 mg |
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) | STAR117D680GA | F;WB | 0.1 mg |
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F;IF;WB | 0.1 mg |
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | Flow | 0.5 mg |
| 文献 |
| 1. Arya, S.K.et al.(2010) Antibody functionalized interdigitated micro-electrode (IDmicroE) based impedimetric cortisol biosensor.Analyst. 135 (8): 1941-6.2. Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA DetectionBiosensors Journal 1: H1106013. Arya, S.K.et al.(2010) Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.Biosens Bioelectron. 25: 2296-301.4. Arya, S.K.et al.(2011) Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor.Biosens Bioelectron. 28 (1): 166-73.5. Kaushik, A.et al.(2013) Mediator and label free estimation of stress biomarker using electrophoretically deposited Ag@AgO-polyaniline hybrid nanocomposite.Biosens Bioelectron. 50: 35-41. |
QQ:1749072012