Mouse anti Human Collagen I
货号：2150-0001
规格：0.1 mg
价格：2406
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Collagen I
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：NFI/20
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human Collagen I, clone NFI/20recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite. Mouse anti human Collagen I, clone NFI/20 does not cross-react with collagen types II, III and IV. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.Mutations in the encoding gene are associated with osteogenesis imperfecta (Penget al.2012), Ehlers Danlos syndrome (Nuytincket al.2000) and idiopathic juvenile osteoporosis (Pococket al.1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihmeet al.1984), |
| 产品详情 | | |
Target Species | Human |
Product Form | Ig fraction - lyophilized |
Preparation | Ig fraction prepared by ammonium sulphate precipitation and DEAE-cellulose chromatography |
Buffer Solution | 0.1M Sodium Chloride 0.01M Sodium Phosphate 0.01M Sodium Borate |
Preservative Stabilisers | 1% Mannitol1% Dextran |
Reconstitution | Use 1 ml of sterile distilled water. |
Immunogen | Human collagen type I from placenta. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/1000 |
Western Blotting | × | | |
Immunohistology - Frozen | √ | | 1/20 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E;WB | 0.5 mg |
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| 文献 |
| 1. Zorin, V.et al.(2015) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.J Tissue Eng Regen Med. 11 (3): 778-86.2. Zorin, V.L.et al.(2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.Biomed Mater. 9 (5): 055005.3. Seedhom, B.B.et al.(2007)In-situengineering of cartilage repair: a pre-clinicalin-vivoexploration of a novel system.Proc Inst Mech Eng H. 221: 475-88. |
技术参数 产品应用 IHC(F);ELISA
产品类型 Monoclonal Antibody
推荐用于 IHC(F);ELISA
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