| 产品介绍 | | |
| Rabbit anti Human collagen I antibodyrecognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
Preparation | Purified IgG prepared by affinity chromatography on immobilized Collagen type I |
Buffer Solution | 0.1M Sodium Chloride0.01M Sodium Phosphate0.01M Sodium Borate |
Preservative Stabilisers | 1% Mannitol1% Dextran |
Reconstitution | Reconstitute with 0.5ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended. |
Immunogen | Collagen type I from human placenta |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/500 | 1/3000 |
Immunohistology - Paraffin1 | √ | 1/10 | 1/50 |
Immunohistology - Frozen | √ | 1/10 | 1/50 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining of paraffin sections.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
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| 数据 |
Published customer image:Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sectionsImage caption:Histological stains after marrow stimulation. Histological stains of cartilage repair tissue collected from 43-year-old patient 8.2 months after marrow stimulation surgery that required revision. Section outlined in blue in H&E stain is fibrocartilage, while the remaining tissue is primarily hyaline-like cartilage. Scale bars = 500 μm. |
| 文献 |
| 1. Evans, M.J.et al.(2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.Am J Physiol Lung Cell Mol Physiol. 283 (6): L1263-70.2. Kim, H.J. & Im, G.I. (2010) The effects of ERK1/2 inhibitor on the chondrogenesis of bone marrow- and adipose tissue-derived multipotent mesenchymal stromal cells.Tissue Eng Part A. 16: 851-60.3. Lee, J.S. and Im, G.I. (2010) Influence of chondrocytes on the chondrogenic differentiation of adipose stem cells.Tissue Eng Part A. 16: 3569-77.4. Kim, Y.J.et al.(2008) PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs.Biochem Biophys Res Commun. 373: 104-85. Li Met al.(2013) Short periods of cyclic mechanical strain enhance triple-supplement directed osteogenesis and bone nodule formation by human embryonic stem cellsin vitro.Tissue Eng Part A. 19 (19-20): 2130-7.6. Hoffman, J.K.et al.(2015) Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.Case Rep Orthop. 2015: 617365. |
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