Native Calf Alkaline Phosphatase
货号：0300-1706
规格：5 mg
价格：2805
产品类型：蛋白
品牌：Bio-Rad
产品介绍 | | |
| Mouse anti Human CD16 antibody, clone LNK16recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996). |
产品详情 | | |
Product Form | Purified AP from calf intestine - liquid |
Preparation | Crystalline suspension. Free of endonuclease, exonuclease and RNase activities. |
Buffer Solution | 70% Ammonium sulphate. |
Preservative Stabilisers | None present |
Activity | 4000 U/mg protein.1 unit liberates 1 µmole of p-nitrophenol per minute at 37ºC and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-nitrophenyl phosphate. |
Approx. Protein Concentrations | Total protein concentration 15.0 mg/ml |
Protein Molecular Weight | 110-150 kDa |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| Store at -20oC only.Storage in frost-free freezers is not recommended.This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Instructions For Use
- The product may be diluted in sterile distilled water or in dilute buffer
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文献 |
| 1.Winkelman, J.et al.(1972) The clinical usefulness of alkaline phosphatase isoenzyme determinations.Am J Clin Pathol. 57 (5): 625-34. |
技术参数 产品应用 ELISA
产品类型 Purified Protein
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