Goat anti Human IgA:Alk. Phos.
货号：205004
规格：1 ml
价格：2635
产品类型：荧光二抗
品牌：Bio-Rad
抗原：IgA
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Alkaline Phosphatase
| 产品介绍 | | |
| Goat anti Human IgA antibodyrecognizes the heavy chain of human IgA as demonstrated by ELISA and Flow cytometry. IgA is the major catagory of antibody present in mucosal secretions and acts as a first line of defense against inhaled or ingested pathogens at the mucosal interface. IgA is also present in serum where it acts to eliminate pathogens via interaction with FcαR, also known as CD89. (Woofet al.2004).Biotinylated Goat anti Human IgA antibody (205008) has been cross-adsorbed against human IgD, IgG and IgM to ensure class specificity. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Alkaline Phosphatase - liquid |
Preparation | Purified IgG prepared by affinity chromatography |
Buffer Solution | TRIS buffered saline, 1mM MgCl2 |
| Antiserum Preparation | Antisera to human IgA were raised by repeated immunisations of goats with highly purified antigen. |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3)50% Glycerol |
Immunogen | Human IgA. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/2000 | 1/4000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls. |
文献 |
| 1. Wright, A.K.et al.(2012) Human Nasal Challenge withStreptococcus pneumoniaeIs Immunising in the Absence of Carriage.PLoS Pathog. 8: e1002622.2. Pennington, S.H.et al.(2016) Polysaccharide-specific Memory B-cells Predict Protection Against Experimental Human Pneumococcal Carriage.Am J Respir Crit Care Med. Jul 12. [Epub ahead of print]3. Turner, P.J.et al.(2020) Differences in nasal immunoglobulin A responses to influenza vaccine strains after live attenuated influenza vaccine (LAIV) immunization in children.Clin Exp Immunol. 199 (2): 109-118.4. Cole, M.E.et al.(2021) Pre-existing influenza-specific nasal IgA or nasal viral infection does not affect live attenuated influenza vaccine immunogenicity in children.Clin Exp Immunol. 204 (1): 125-133. |
技术参数 产品应用 ELISA
产品类型 Polyclonal Antibody
推荐用于 ELISA
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