Mouse IgG1 Negative Control:Low Endotoxin
货号：MCA1209EL
规格：0.5 mg
价格：4063
产品类型：小鼠同型对照
品牌：Bio-Rad
物种：其它
宿主：小鼠
抗体亚型：IgG1
荧光染料：Low Endotoxin
| 产品介绍 | | |
| Mouse IgG1 Negative Control antibodyis suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES |
| 产品详情 | | |
Target Species | Negative Control |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Immunogen | Human T lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Endotoxin Level | <0.01 EU/ug |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20oC only.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.Use 10ul of the suggested working dilution to label 106cells in 100ul |
| 文献 |
| 1. Weiss, D.J.et al.(2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate ofMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.avium.J Leukoc Biol. 83 (1): 48-55.2. Chen, W.et al.(2009) Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis.Mol Vis. 15: 619-28.3. Safeukui Iet al.(2015) Malaria induces anemia through CD8+ T cell-dependent parasite clearance and erythrocyte removal in the spleen.MBio. 6 (1) pii: e02493-14.4. Aricha, R.et al.(2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.J Autoimmun. 67: 57-64.5. Wattegedera, S.R.et al.(2017) Enhancing the toolbox to study IL-17A in cattle and sheep.Vet Res. 48 (1): 20.6. Stangl, H.et al.(2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.Sci Rep. 10 (1): 17071. |
技术参数 产品应用 Flow
产品类型 Negative/Isotype Control
推荐用于 Flow
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