TRA-1-60 Monoclonal Antibody (TRA-1-60)

货号：MA1023,MA1023X

规格：100μg,20 µL

价格：4070,2148

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-60

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1:500-1:2,000 (WB)；1:20-1:100(ICC/IF)；0.4 µg (Flow)

靶标信息TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

数据

Immunofluorescent analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product## MA1-023) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product## 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing TRA-1-60 (Product## MA1-023), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with a fluorescent goat-anti-mouse IgM secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product## 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

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