| 产品介绍 | | |
| Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012) , lapine (Pakandl et al, 2008) and guinea-pig tissues.This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. |
| 产品详情 | | |
Target Species | Negative Control |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid. |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Max Ex/Em | 490/525 |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
| 数据 |
| Figure A. RPE conjugated Mouse anti Bovine CD28 (MCA5779PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD28 (MCA5779PE) and FITC conjugated Mouse anti Bovine CD44 (MCA2433F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells. |
| 文献 |
| 1. Kamble, N.M.et al.(2016) Interaction of a live attenuatedSalmonella gallinarumvaccine candidate with chicken bone marrow-derived dendritic cells.Avian Pathol. Jan 26:1-24. [Epub ahead of print]2. Brace, P.T.et al.(2017)Mycobacterium tuberculosissubverts negative regulatory pathways in human macrophages to drive immunopathology.PLoS Pathog. 13 (6): e1006367.3. Topoluk, N.et al.(2017) Amniotic Mesenchymal Stromal Cells Exhibit Preferential Osteogenic and Chondrogenic Differentiation and Enhanced Matrix Production Compared With Adipose Mesenchymal Stromal Cells.Am J Sports Med. 363546517706138.4. Iwaszko-Simonik, A.et al.(2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).Vet Immunol Immunopathol. 164 (1-2): 87-92.5. Arzi, B.et al.(2017) Therapeutic Efficacy of Fresh, Allogeneic Mesenchymal Stem Cells for Severe Refractory Feline Chronic Gingivostomatitis.Stem Cells Transl Med. 6 (8): 1710-22.6. Taechangam, N.et al.(2021) Feline adipose-derived mesenchymal stem cells induce effector phenotype and enhance cytolytic function of CD8+ T cells.Stem Cell Res Ther. 12 (1): 495. |
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