| 产品介绍 | | |
| Mouse anti Human CD8 antibody, clone LT8recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells.The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to StarBright UltraViolet 400 - liquid |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Marmoset | √ |
| Chimpanzee | √ |
| Cynomolgus monkey | √ |
| Red-bellied Tamarin | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Preparation | Purified IgG prepared by ion exchange chromatography |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 0.1% Pluronic F68 0.1% PEG 3350 0.05% Tween 20 |
Immunogen | Normal human peripheral blood lymphocytes. |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
StarBright UltraViolet 400 | 347 | 394 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4°C. DO NOT FREEZE.This product should be stored undiluted. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 数据 |
| Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright UltraViolet 400 conjugated Mouse anti Human CD8 (MCA1226SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. |
| 文献 |
| 1. Nelson, M.et al.(2010) Characterization of lethal inhalational infection withFrancisella tularensisin the common marmoset (Callithrix jacchus).J Med Microbiol. 59: 1107-13.2. Gibbings, D.J.et al.(2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.BMC Immunol. 8: 12.3. Junker, A.et al.(2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.Brain. 130: 2789-99.4. Held, K.et al.(2011) Expression of herpes simplex virus 1-encoded microRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates.J Virol. 85 (19): 9680-5.5. Hood SPet al.(2014) Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).Virus Res. 179: 93-101.6. Nelson, M. & Loveday, M. (2014) Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset.J Immunol Res. 2014: 913632.7. Manivannan, K.et al.(2016) CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.PLoS Pathog. 12 (4): e1005560.8. Gross, C.C.et al.(2016) Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation.Proc Natl Acad Sci U S A. 113 (21): E2973-82.9. Dunham, J.et al.(2016) Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.J Neuroimmune Pharmacol. 11 (1): 73-83.10. Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088.11. Philippens, I.H.et al.(2017) Acceleration of Amyloidosis byInflammation in the Amyloid-Beta Marmoset Monkey Model of Alzheimer's Disease.J Alzheimers Dis. 55 (1): 101-113. |
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