FOXP3 Monoclonal Antibody (PCH101), APC, eBioscience™

货号： 17-4776-41, 17-4776-42

规格：25 tests,100 tests

价格：2372,4340

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：PCH101

荧光染料：APC

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	5 µL (0.5 µg)/test(Flow)

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer and protocol. Refer to Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationFOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

FOXP3 Antibody (17-4776-41) in FlowSurface staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) followed by intracellular staining with Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or Anti-Human Foxp3 APC (right) using Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00). FOXP3 Antibody (17-4776-41)Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, Foxp3 clone PCH101 stains a subset of the CD4+ T cells and does not stain the CD8+ T cells. Details: Normal human peripheral blood cells were surface stained with CD3 (clone UCHT1), CD4 (clone RPA-T4, top), and CD8 (clone OKT8, bottom), followed by intracellular staining with Rat IgG2a kappa Isotype Control (left) or Foxp3 (clone PCH101, right) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD3+ gate were used for analysis. Relative expression validation info.

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参考文献：

1.Journal of virology Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1. "17-4776 was used in Flow cytometry/Cell sorting to show that follicular regulatory T cells are highly permissive to HIV-1 both ex vivo and in vivo." AuthorsMiller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E 2.Clinical and experimental immunology survival and CTLA-4-dependent function in autoimmune liver diseases. "17477642 was used in flow cytometry to examine the potential of IL-2 to enhance T regulatory cell therapy" AuthorsJeffery HC,Jeffery LE,Lutz P,Corrigan M,Webb GJ,Hirschfield GM,Adams DH,Oo YH 3.Experimental and therapeutic medicine Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance. 4.PloS one Paramyxovirus infection regulates T cell responses by BDCA-1+ and BDCA-3+ myeloid dendritic cells. "17-4776 was used in Immunocytochemistry to demonstrate a virus-specific and subset specific effect of RSV and hMPV infection on mDC function." AuthorsGupta MR,Kolli D,Molteni C,Casola A,Garofalo RP 5.BMC infectious diseases The number of CCR5 expressing CD4+ T lymphocytes is lower in HIV-infected long-term non-progressors with viral control compared to normal progressors: a cross-sectional study. "17-4776 was used in Immunohistochemistry to determine levels of CCR5 and CXCR4 expression among CD4(+) and CD8(+) lymphocyte subsets in HIV-infected long-term non-progressors." AuthorsMeijerink H,Indrati AR,van Crevel R,Joosten I,Koenen H,van der Ven AJ

技术参数

产品应用 Flow；ICC；IF；IHC；MISC；IHC (P)

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