HemaTox™ Myeloid Kit/HemaTox™髓系分化检测试剂盒-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

HemaTox™ Myeloid Kit/HemaTox™髓系分化检测试剂盒

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HemaTox™ Myeloid Kit/HemaTox™髓系分化检测试剂盒

货号：09704

规格：1kit

价格：11114

产品类型：造血干细胞培养

品牌：STEMCELL Technologies

HemaTox™ Myeloid Kit is intended for the measurement of drug toxicity on the proliferation and myeloid-specific differentiation of human hematopoietic stem and progenitor cells (HSPCs) in a liquid culture-based 96-well plate format. HemaTox™ Myeloid Kit includes a specialized serum-free culture medium and 100X supplement. Complete HemaTox™ Myeloid Medium (HemaTox™ Myeloid Medium + HemaTox™ Myeloid 100X Supplement) promotes the proliferation of human CD34+ HSPCs and their differentiation into myeloid cells during a 7-day culture period. After culture, the cells can be counted and assessed for expression of myeloid markers such as CD13, CD14, and CD15 using flow cytometry or other methods.HemaTox™ Myeloid Kit may be used on its own or in combination with HemaTox™ Erythroid Kit (Catalog #09701) or HemaTox™ Megakaryocyte Kit (Catalog #09707) to assess lineage-specific drug toxicity in parallel.Each kit contains sufficient medium and supplement for testing up to 160 different conditions (triplicate wells per condition, 200 µL per well) in 5 x 96-well plates.HemaTox™髓系试剂盒用于在基于液体培养基的96孔板中测定药物对人造血干细胞和祖细胞（HSPCs）的增殖和髓系特异性分化的毒性。 HemaTox™髓系试剂盒包括一个专门的无血清培养基和100X补充剂。完整的HemaTox™髓样培养基（HemaTox™髓样培养基+ HemaTox™髓样100X增补剂）可促进人类CD34 + HSPCs的扩增，并在培养7天的过程中分化为髓样细胞。培养后，可用流式仪或其他方法对细胞进行计数和评估骨髓标志物（如CD13，CD14和CD15）的表达。HemaTox™髓系试剂盒可单独使用或与HemaTox™Erythroid试剂盒（货号＃09701）或HemaTox™巨核细胞试剂盒（货号＃09707），同时评估谱系特异性药物毒性。每个试剂盒含有足够的培养基和补充剂，用于检测多达160种不同条件（每种条件一式三份，每孔200μL） ×96孔板。

优点：

- 体系已验证.IC50值类似于CFU 的实验结果，CFU目前是体外血液毒性检测实验的金标准。- 高特异性. 在使用此试剂盒培养的条件下，可以将CD34+ HPSC特异性地分化成红系，髓系及巨核祖细胞。- 使用灵活. 待测的化合物可以在培养起始时添加来研究对分化前祖细胞的影响，也可以在培养稍后阶段添加来研究化合物对分化细胞的影响。读数方法多样，可以通过流式细胞仪，也可以通过读板机。

数据：

Figure 1.General HemaTox™ Myeloid Kit Procedure*The cell isolation step may be omitted if pre-enriched CD34+ cells are used.图1.一般HemaTox™髓系试剂盒使用流程如果使用预富集的CD34⁺细胞，可以省略细胞分离步骤。

Figure 2.Flow Cytometry Plot Showing Cells Produced After Culture of CD34+ HSPCs with the HemaTox™ Myeloid Kit(A) Human CB CD34+ cells were cultured with the HemaTox™ Myeloid Kit using the protocol as written in the Product Information Sheet (PIS). (B) After the appropriate culture period, cells were harvested and stained for cell surface proteins expressed on myeloid (CD13 and CD15) cells.

图2.用HemaTox™髓样试剂盒培养CD34 + HSPCs后，产生细胞的流式分析图（A）使用产品信息表（PIS）中记录的方案，用HemaTox™髓系试剂盒培养人CB CD34 +细胞。（B）在适当的培养期后，收获细胞并对在骨髓（CD13和CD15）细胞上表达的细胞表面蛋白进行染色。

Figure 3.Reproducibility of HemaTox™ Myeloid Kit Results Between Experiments and Using Different CD34+ Cell Preparations(A) Dose-response curves were generated from titrations of 5-FU added to human CB CD34+ cells isolated from 3 donors and cultured with the HemaTox™ Myeloid Kit. Three to five separate experiments were performed with cells from each donor. In each assay similar IC50 values were obtained with cells from different donors and in different experiments with cells from the same donor. Shown are values normalized to the percentages (%) of maximum cell growth without drug. Despite differences in absolute cell counts, curves are reproducible when normalized within each experiment. (B) Table showing IC50 values generated for 5-FU in culture with the myeloid kit including standard deviation (SD) and the coefficient of variation (% CV) across experiments.图3. HemaTox™髓样试剂盒在实验和使用不同CD34 +细胞制剂之间的重复性（A）剂量 - 反应曲线通过添加到从3个供体分离的人CB CD34⁺细胞中的5-FU的滴定产生，并用HemaTox™髓样试剂盒培养。用来自每个供体的细胞进行3-5次单独的实验。在每个测定中，来自不同供体的细胞和来自相同供体的细胞的不同实验中获得相似的IC 50值。显示的是标准化为没有药物的最大细胞生长百分比（％）的值。尽管绝对细胞计数存在差异，但是在每个实验中归一化时曲线是可重复的。（B）该表显示了在包含标准偏差（SD）和变异系数（％CV）的髓样试剂盒培养物中5-FU产生的IC50值。

Figure 4.Correlation Between IC50 Values for Six Drugs Measured Using the CFU-GM Assay and the 96-Well Plate Liquid Culture-Based HemaTox™ Myeloid KitHuman BM CD34+ cells were cultured in colony-forming unit - granulocyte/macrophage (CFU-GM) assays with MethoCult™ medium and in liquid culture with the HemaTox™ Myeloid Kit. IC50 values generated using each assay were plotted on the X and Y axes and shown to be highly correlated with a correlation coefficient (R2) of 0.91.

图4.使用CFU-GM测定和96孔板液体培养基的HemaTox™髓系试剂盒测量的六种药物的IC50值之间的相关性使用MethoCult™培养基和HemaTox™髓样试剂盒进行液体培养，在集落形成单位 - 粒细胞/巨噬细胞（CFU-GM）测定中培养人类BM CD34 +细胞。使用每个测定产生的IC 50值绘制在X和Y轴上，并显示与0.91的相关系数（R 2）高度相关。

Figure 5.Lineage-Specific Differences in Hematotoxicity Identified with HemaTox™ Erythroid, Myeloid and Megakaryocyte KitsResults show average IC50 values for each drug tested on human BM CD34⁺ cells using the HemaTox™ Erythroid (grey), Myeloid (gold) and Megakaryocyte (orange) Kits. Most drugs show similar toxicity for each lineage but some, such as Sunitinib, are ~100-fold more toxic for erythroid than for megakaryocyte progenitor differentiation with intermediate toxicity for myeloid progenitor differentiation. Vertical lines indicate standard error of the mean (SEM) (n = 4 - 8).图5.用HemaTox™红细胞、髓样和巨核细胞试剂盒鉴定的血细胞特异性差异使用HemaTox TM红细胞（灰色）、骨髓（金）和巨核细胞（橙）试剂盒在人BM CD34 +细胞上测试的结果显示每种药物的平均IC 50值。大多数药物对各种谱系显示相似的毒性，但是一些（例如Sunitinib）对于红细胞的毒性比对巨核细胞祖细胞分化具有中毒的髓细胞祖细胞分化大约100倍以上。垂直线表示平均值的标准误差（SEM）（n = 4-8）。

组成成分：

▪HemaTox™ Myeloid Medium, 100 mL▪HemaTox™ Myeloid 100X Supplement, 1 mL ▪ HemaTox™髓样培养基，100 mL▪ HemaTox™髓样100X添加物，1 mL

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