Anti-Mouse MHC Class II (I-A/I-E) APC

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Anti-Mouse MHC Class II (I-A/I-E) APC

货号:86212-80-25,86212-80-100

规格:25 μg,100 μg

价格:542,1760

产品类型:流式抗体

品牌:Biogems

物种:人

宿主:小鼠

抗体亚型:IgG

荧光染料:Purified

Catalogue Number :86212-80

Description

The M5/114.15.2 monoclonal antibody or (M5/114) is specific for the mouse major histocompatibility complex class II, specific for the I-A and I-E subregion-encoded glycoproteins (alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, but not the I-Af, I-Ak, nor I-As antigens). The antibody also reacts with cells from the H-2p or H-2r haplotypes mice.

The polymorphic determinant represented by the mouse major histocompatibility complex class II (MHC Class II) is expressed by the immune cells bearing the specified alloantigens, like T and B lymphocytes, monocytes, dendritic cells, and macrophages. The antibody doesn’t react with the NOD H-2g7 haplotype alloantigens.

M5/114 mAb inhibits I-A-restricted T cell responses of the Haplotypes H-2b, H-2d, H-2q, and H-2u.

C57Bl/6 splenocytes were stained with APC M5/114.15.2 with relevant isotype control in Red.

Additional Information

Clone

M5/114.15.2

Format:

APC

Applications

FC

Reactivity

Mouse

Isotype:

Rat IgG2b, kappa

Research Interest:Innate Immunity,Adaptive Immunity
Cell Type:B Cells,Monocytes and Macrophages,T Cells

Preperation:

The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Formulation:

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

References:

Bhattacharya, A. L. O. K., Dorf, M. E., & Springer, T. A. (1981). A shared alloantigenic determinant on Ia antigens encoded by the IA and IE subregions: evidence for I region gene duplication.The Journal of Immunology,127(6), 2488-2495.

Mendiratta, S. K., Singh, N., Bal, V., & Rath, S. (1996). Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity.Immunology,89(2), 238-244.

Unternaehrer, J. J., Chow, A., Pypaert, M., Inaba, K., & Mellman, I. (2007). The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface.Proceedings of the National Academy of Sciences,104(1), 234-239.

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