CD279 (PD-1) Monoclonal Antibody (J43), FITC
货号：11-9985-82,11-9985-81,11-9985-85
规格：100 μg,50 μg,500 μg
价格：1972,1205,3041
产品类型：流式抗体
品牌：eBioscience
物种：小鼠
宿主：仓鼠
抗体亚型：IgG
荧光染料：FITC
| Tested Species reactivity | Mouse |
| Published species reactivity | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control, FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J43 |
| Conjugate | FITC |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.1% gelatin |
| Contains | 0.09% sodium azide |
| Storage conditions | 4° C, store in dark, DO NOT FREEZE! |
| RRID | AB_465472 |
Product Specific Information
Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis.Applications Tested: The J43 antibody has been tested by flow cytometric analysis of Con-A activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.
Background/Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of ""danger signals"" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
数据：
Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 µg of Anti-Mouse CD279 (PD-1) FITC. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
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