| 产品介绍 | | |
| Mouse anti Human CD227 antibody, clone VU-4H5recognizes human Mucin 1, also known as CD227, Cancer antigen 15-3, Episialin or Krebs von den Lungen-6. CD227 is a 1255 amino acid single pass type I transmembrane glycoprotein. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti Human CD227 antibody, clone VU-4H5 reacts with the APDTR peptide sequence (VTSAPDTRPAPGSTAPPAHG) of human CD227. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunoge | 60-mer tandem repeat of VTSAPDTRPAPGSTAPPAHG, conjugated to BSA. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| Immunohistology - Paraffin | √ | | |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5mg |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E,WB | 0.5mg |
| 文献 |
| 1.Treon, S.P.et al.(1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.Blood. 93: 1287-98.2.Karsten, U.et al.(2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).Glycobiology. 14: 681-92.3.Treon, S.P.et al.(2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.Blood. 96: 3147-53.4.Singh, R.et al.(2007) Target-specific cytotoxic activity of recombinant immunotoxin scFv(MUC1)-ETA on breast carcinoma cells and primary breast tumors.Mol Cancer Ther. 6: 562-9.5.Vlad, A.M.et al.(2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.J Exp Med. 196: 1435-46.6.ten Berge, R.L.et al.(2001) MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.J Clin Pathol. 54: 933-9. |
QQ:1749072012