CD279 (PD-1) Monoclonal Antibody (MIH4), PE
货号：12-9969-41,12-9969-42
规格：25 Tests,100 Tests
价格：1492,2773
产品类型：流式抗体
品牌：eBioscience
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PE
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.5 μg)/test (Flow) |
| 产品详细信息Description: The MIH4 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The MIH4 antibody recognizes a different epitope than antibody clones J105.Applications Reported: This MIH4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MIH4 antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (12-9969-41) in FlowStaining of 3-day PHA-stimulated human peripheral blood cells with Anti-Human CD25 APC (Product # 17-0259-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD279 (PD-1) PE (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.PloS oneHIV-induced T-cell activation/exhaustion in rectal mucosa is controlled only partially by antiretroviral treatment."12-9969 was used in Flow cytometry/Cell sorting to investigate the effect of chronic HIV infection on the immune cell phenotype."2.Nature medicineImmunogenic neoantigens derived from gene fusions stimulate T cell responses."Published figure using CD279 (PD-1) monoclonal antibody (Product # 12-9969-42) in Flow Cytometry"3.Cancer researchA mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells."12-9969 was used in Flow cytometry/Cell sorting to investigate whether exposure of mouse and human tumour cells to hypoxia increases their resistance to T cell-mediated immunity."4.Arthritis research and therapyEnhanced IL-6/phosphorylated STAT3 signaling is related to the imbalance of circulating T follicular helper/T follicular regulatory cells in patients with rheumatoid arthritis."12-9969 was used in Flow cytometry/Cell sorting to demonstrate that enhanced IL-6/pSTAT3 signalling may contribute to promotion of Tfh cells, consequently skewing the ratio of Tfh to Tfr cells, which may be crucial for disease progression in rheumatoid arthritis.5.Experimental and therapeutic medicineIncreased levels of CCR7(lo)PD-1(hi) CXCR5+CD4+T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure."Published figure using CD279 (PD-1) monoclonal antibody (Product # 12-9969-42) in Flow Cytometry" |
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