货号：108118

规格：100µg

价格：2730

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：Purified

Description

Ly-6A/E,also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

• Isotype Control

• Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl

• Reactivity

• Mouse

• Immunogen

• IL-2-dependent mouse T-cell line (CTL-L)

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.

• Concentration

• 0.5 mg/ml

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality testedIHC, IF - Reported in the literature

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

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• Excitation Laser

• Red Laser (633 nm)

• Application Notes

• The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1,in vitrolymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13and Bouin-fixed,paraffin-embedded samples9.The two Sca-1 recognizing clonesD7andE13-161.7have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14

• Application References(PubMed link indicates BioLegend citation)

• 1. Ortega G,et al.1986.J. Immunol.137:3240. (WB, IP)2. Palfree RGE,et al.1986.Immunogenetics23:197. (WB)3. Codias EK,et al.1990.J. Immunol.144:2197.4. Malek TR,et al.1986.J. Exp. Med.164:709.5. Codias EK,et al.1990.J. Immunol.145:1407.6. Ivanov V,et al.1994.J. Immunol.153:2394.7. Karlhofer FM,et al.1991.J. Immunol.146:3662.8. Fleming T,et al.1994.J. Immunol.153:1955.9. van Bragt MPA,et al.2005.Biol. Reprod.73:634. (IF, IHC)10. Umland O,et al.2007.J. Immunol.178:4147.11. Cridland SO,et al.2009.Blood Cell. Mol. Dis.45:149. (FC)PubMed12. Pronk CJ,et al.2011.J. Exp Med.PubMed13. English A,et al. 2000.J. Immunol. 165:3763. (IHC)14. Bamezai A and Rock KL. 1995.Proc. Natl. Acad. Sci. USA92:4294.