Mouse anti Human CD49b antibody, clone 31H4
货号：MCA2027
规格：0.2 mg
价格：3783
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD49b
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：31H4
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human CD49b antibody, clone 31H4recognizes human CD49b also known as integrin alpha-2, VLA-2 subunit alpha or collogen receptor. CD49b is a 1181 amino acid ¦130 kDa single pass type-1 transmembrane glycoprotein which forms a heterodimer with integrin beta-1.Mouse anti Human CD49b antibody, clone 31H4 has been identified as being capable of immunoprecipitating the integrin a2 subunit from human cell-lines surface labelled with125I. It has been confirmed specific to a2 by relative expression of antigen on various cell lines by flow cytometry, and recognition of affinity purified a2b1 in dot blots. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract. |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Tanaka, T.et al.(2009) Remodeling of the human endometrial epithelium is regulated by laminin and type IV collagen.Int J Mol Med. 23: 173-80.2.Tanaka, T.et al.(2005) Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells.Oncol Rep. 14: 941-8.3.Tanaka, T.et al.(2010) Establishment and characterization of novel human uterine leiomyosarcoma cell linesInt J Oncol. 37: 125-31.4.Tanaka, T.et al.(2012) Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells.Int J Oncol. 41: 1837-44. |
技术参数 产品应用 Elisa；Flow
产品类型 Monoclonal Antibody
推荐用于 Elisa；Flow
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