ZR Plasmid Miniprep-Classic Kit, 800 Preps/Unit
货号：D4016
规格： 800 Preps
价格：3520
产品类型：DNA提取
品牌：Zymo
The ZR Plasmid Miniprep™-Classic is designed for efficient isolation of plasmid DNA fromE. Coliusing a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a uniqueFast-Spincolumn to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. The innovative Zymo-Spin™ llN columns yield endotoxin-free plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep™-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
产品特点
- For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, - DNA sequencing, transformation, cloning, transfection, in vitro transcription reactions, etc.
- Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps.
- Unique column design: zero buffer retention and low (30 µl) elution volume.
组成成份
| Product | Size | Catalog # |
|---|
| Buffer P1 (80 ml) (Red) | 80 ml | D4027-1-80 |
| Buffer P2 (80 ml) (Green) | 80 ml | D4027-2-80 |
| Buffer P3 (220 ml) (Yellow) | 220 ml | D4027-3-220 |
| Endo-Wash Buffer (60 ml) | 60 ml | D4036-3-60 |
| Plasmid Wash Buffer (concentrate) (48 ml) | 48 ml | D4027-4-48 |
| DNA Elution Buffer | 16 ml | D3004-4-16 |
| Zymo-Spin™ IIN | 50 Pack | C1019-50 |
| Collection Tubes (2 ml) | 50 Pack | C1001-50 |
实验数据
Plasmid products.Restriction Endonuclease digestion of three different plasmids prepared using the ZR Plasmid Miniprep™-Classic, performed in triplicate. M: ZR 1 kb DNA marker (Zymo Research)
Sequence-quality DNA preparations.DNA sequencing chromatogram of plasmid DNA prepared using the ZR Plasmid Miniprep™-Classic.
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