货号：115940

规格：100µg

价格：3850

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：APC

Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

• Isotype Control

• APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl

• Reactivity

• Mouse

• Immunogen

• Mouse SLAM-human IgG1 fusion protein

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

• Application Notes

• The TC15-12F12.2 antibody has been reported to enhance the production of IFN-γ by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion1, enhancing IFN-γ production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 115906).

• Application References(PubMed link indicates BioLegend citation)

• 1. Castro AG,et al.1999.J. Immunol.163:5860. (FC, Costim, IP)2. Forsberg EC,et al.2005.PLoS Genet.1:e28. (FC)3. Terrazas LI,et al.2005.Int. J. Parasitol.35:1349. (FC)4. Cannons JL,et al.2006.J. Exp. Med.203:1551. (FC)5. Umemoto T,et al.2006.J. Immunol.177:7733. (FC)6. Jordan MA,et al.2007.J. Immunol.178:1618. (FC, Block)PubMed7. Jung Y,et al.2007.Blood110:82.PubMed8. Pimanda JE,et al.2007.Proc. Natl. Acad. Sci. USA104:840.9. Sugiyama T,et al.2007.Proc. Natl. Acad. Sci. USA104:175.10. Kim I,et al.2006.Blood108:737.PubMed11. Ema H,et al.2006.Nat Protoc.1:2979.PubMed12. Fraser ST,et al.2007.Blood109:4616.PubMed13. Jung Y,et al.2008.Stem Cells.26:2042.Pubmed14. Song J,et al.2010.Blood115:2592.PubMed15. Cridland SO,et al.2009.Blood Cell. Mol. Dis.43:149. (FC)PubMed16. Morita Y,et al.2010.J. Exp Med.207:1173.PubMed