货号：103437

规格：25µg

价格：1610

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：PE

Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

• Isotype Control

• PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl

• Reactivity

• Mouse

• Immunogen

• Mouse T lymphoma MBL-2

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

• Excitation Laser

• Blue Laser (488 nm)

• Application Notes

• The HM48-1 antibody is useful for blockingin vitroandin vivoCD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103408). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103430) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

• Application References(PubMed link indicates BioLegend citation)

• 1. Kato K,et al.1992.J. Exp. Med.176:1241. (IP, Costim, Block)2. Qin L,et al.1994.J. Exp. Med.179:341. (Costim, Block)