货号：101328

规格：100µg

价格：3850

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：APC

Description

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

• Reactivity

• Mouse

• Immunogen

• Sorted pre-B cells

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

• Excitation Laser

• Red Laser (633 nm)

• Application Notes

• Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (≤1.0 µg per 106cells in 100 µl volume) for 5-10 minutes on ice prior to immunostaining. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101310). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

• Application References(PubMed link indicates BioLegend citation)

• 1. Personal communication (IP)2. Oliver AM,et al.1999.Hybridoma18:113. (Block)3. Brummel R and Lenert P. 2005.J. Immunol.174:2429.4. Terrazas LI,et al.2005.Int. J. Parasitol.35:1349. (Block)5. Clements JL,et al.2006.J. Immunol.177:905.6. Mohamed W,et al.2010.Infect Immun.78:3306.PubMed7. Ouchi T,et al.2011.J. Exp Med.208:2607.PubMed8. Kmieciak M,et al.2011.J. Vis. Exp.47:2381.PubMed9. Yamazaki S,et al.2012.PLoS One.7:e51665.PubMed10. Li J,et al.2012.Arthritis Rheum.64:1098.PubMed11. Azuma M,et al.2012.Oncoimmunology.1:581.PubMed12. Koon HW,et al.2013.J. Vis. Exp.68:4208.PubMed13. Hegde VL,et al.2013.J Biol Chem.288:36810.PubMed14. Huang J,et al.2013.J. Immunol Methods.387:254.PubMed15. Dutow P,et al.2014.J Infect Dis.PubMed16. Fan Y,et al.2014.J Exp Med.211:313.PubMed17. Huang HN,et al.2014.Antimicrob Agents Chemother.58:1538.PubMed18. Takei S,et al.2014.Vaccine.32:3066.PubMed19. Richardson ML,et al.2014.PLoS Negl Trop Dis.8:2825.PubMed20. Cekanaviciute E,et al.2014.J Immunol.193:139.PubMed21. Kimura T,et al.2014.Int Immunol.26:697.PubMed22. Everad A,et al.2014.Nat Commun.5:5648.PubMed23. Cenci E,et al.2006.J. Leuko. Biol.79(1):40-5. (Block)