货号：640908

规格：100 tests

价格：2590

产品类型：流式抗体

品牌：Biolegend

物种：人

宿主：仓鼠

抗体亚型：IgG

荧光染料：PE

Description

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (Cat. # 422201) is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (Cat. #420403/420404) or Propidium Iodide Solution (Cat. # 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

• Reactivity

• All mammalian species

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

• Preparation

• The purified protein was conjugated with PE under optimal conditions.

• Concentration

• Lot-specific (please contacttechnical supportfor concentration and total µg amount, or use ourLookuptool if you have a lot number.)

• Storage & Handling

• The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

• Excitation Laser

• Blue Laser (488 nm)

• Application Notes

• Annexin V Staining1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. # 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. # 422201) at a concentration of 1x106cells/ml.2. Transfer 100 µl of cell suspension in 5 ml test tube.3. Add 5 µl of PE Annexin V.4. Add 10 µl of PI solution (Cat. # 421301) or 7-AAD (Cat. #420403/420404).5. Gently vortex the cells, and incubate for 15 min at room temperature (25°C), in the dark.6. Add 400 µl of Annexin V Binding Buffer (Cat. # 422201) to each tube. Analyze by flow cytometry.

• Application References(PubMed link indicates BioLegend citation)

• 1. Koopman G, et al. 1994.Blood84:1415.2. Vermes I, et al. 1995.J. Immunol. Methods184:39.3. Dachary-Prigent J, et al. 1993.Blood81:2554.4. Sekine C,et al.2009.Int Immunol.PubMed5. Grujic M,et al.2010.J. Immunol.185:1730.PubMed