CD7 Monoclonal Antibody (4H9), FITC, eBioscience™
货号：11-0078-41,11-0078-42
规格：25 tests,100 tests
价格：0,2271
产品类型：流式抗体
品牌：eBioscience
抗原：CD7
物种：人
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：4H9
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| 浓度： | 5 µL/Test | 用法： | 5 µL (0.125 µg)/test (Flow） |
| 产品详细信息Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation. CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma.Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Expression of CD7 is an important marker used in leukemia diagnostics. |
| 数据 |
| CD7 Antibody (11-0078-41) in Flow Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0048-42) and Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD7 FITC (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Leukemia Casein kinase 2 controls the survival of normal thymic and leukemic γδ T cells via promotion of AKT signaling.2.Nature communications Trained immunity in newborn infants of HBV-infected mothers.3.PLoS pathogens Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.4.Journal of immunology (Baltimore, Md. : 1950) IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.5.Blood A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies. |
QQ:1749072012