货号：359310

规格：100 tests

价格：3570

产品类型：流式抗体

品牌：Biolegend

物种：人

宿主：仓鼠

抗体亚型：IgG

荧光染料：APC

Description

CD49b is a 170 kD transmembrane protein, also known as α2integrin, VLA-2 α chain, Integrin α2and GPIa. It associates with CD29 (β1integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.

• Isotype Control

• APC Mouse IgG1, κ Isotype Ctrl (FC)

• Reactivity

• Human

• Immunogen

• HT1080 cells

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

• Preparation

• The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

• Concentration

• Lot-specific (please contacttechnical supportfor concentration and total µg amount, or use ourLookuptool if you have a lot number.)

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

• Excitation Laser

• Red Laser (633 nm)

• Application Notes

• Additional reported applications (for the relevant formats of this clone) include:in vitroblocking activity1,2, immunoprecipitation3, and ELISA4. The LEAF™ purifiedantibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered)is recommended for functional studies (Cat. No. 359304).

• Application References(PubMed link indicates BioLegend citation)

• 1. Hirsch MS,et al.1997.Dev. Dyn.210:249. (Block)2. Sawhney RS,et al.2006.J. Biol. Chem.281:8497. (Block)3. Lee SA,et al.2009.Carcinogensis.30:1872. (IP)4. Zárate S,et al.2004.J. Virol.78:10839. (ELISA)