| The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.Applications Reported: The MB19-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered. |
| 1. Journal of immunology (Baltimore, Md. : 1950) Inflammatory spleen monocytes can upregulate CD11c expression without converting into dendritic cells."13-0191 was used in Magnetic cell separation to establish the contribution of inflammatory DCs (iDCs) and conventional DCs (cDCs) in response to anti-CD40 administration in mice."Authors Drutman SB,Kendall JC,Trombetta ES2. Journal of immunology (Baltimore, Md. : 1950) Differential geminin requirement for proliferation of thymocytes and mature T cells."13-0191 was used in Magnetic cell separation to gain insight into the in vivo role of Geminin in progenitor cell division and differentiation."Authors Karamitros D,Kotantaki P,Lygerou Z,Veiga-Fernandes H,Pachnis V,Kioussis D,Taraviras S3. Journal of immunology (Baltimore, Md. : 1950) Dendritic cells continue to capture and present antigens after maturation in vivo."13-0191 was used in Magnetic cell separation to study the dynamics of Ag capture and presentation in situ, showing that dendritic cells continue to capture and present antigens after maturation."Authors Drutman SB,Trombetta ES4. The Journal of clinical investigation Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice."13-0191 was used in Flow cytometry/Cell sorting to reveal a crucial role for IMs in maintaining immune homeostasis in the respiratory tract."Authors Bedoret D,Wallemacq H,Marichal T,Desmet C,Quesada Calvo F,Henry E,Closset R,Dewals B,Thielen C,Gustin P,de Leval L,Van Rooijen N,Le Moine A,Vanderplasschen A,Cataldo D,Drion PV,Moser M,Lekeux P,Bureau F5. Journal of immunology (Baltimore, Md. : 1950) E47 controls the developmental integrity and cell cycle quiescence of multipotential hematopoietic progenitors."13-0191 was used in Flow cytometry/Cell sorting to show E47 controls the developmental integrity and cell cycle quiescence of hematopoietic progenitors."Authors Yang Q,Kardava L,St Leger A,Martincic K,Varnum-Finney B,Bernstein ID,Milcarek C,Borghesi L6.Genes and development Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling."13-0191 was used in Flow cytometry/Cell sorting to study how Notch/Delta signaling is not sufficient for T-lineage specification and commitment, but can be permissive for the maintenance and proliferation of uncommitted progenitors."Authors Taghon TN,David ES,Zúñiga-Pflücker JC,Rothenberg EV |
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