ANTI-MOUSE CD19 (MB19-1) FG PURIFIE
货号:16-0191-85
规格:FG PURIFIE
价格:3779
产品类型:流式抗体
品牌:eBioscience
抗原:CD19
物种:小鼠
宿主:小鼠
抗体亚型:IgG
克隆号:MB19-1
荧光染料:Fg Purified
抗体类型: | 单抗 | 同型对照: | IgG2a, kappa |
免疫原性: | | 应用领域: | Flow;FN;IP |
推荐用法: | 2µg/test(flow) |
The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.Applications Reported: This MB19-1 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. The Functional Grade MB19-1 can also be used in functional assays.Applications Tested: This MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC. |
数据 |
CD19 Antibody (16-0191-85)Staining of C57Bl/6 splenocytes with 1 µg of Mouse IgA Isotype Control Functional Grade Purified (Product # 16-4762) (open histogram) or 1 µg of Anti-Mouse CD19 Functional Grade Purified (filled histogram) followed by Anti-Mouse IgA FITC (Product # 11-4204-83). Cells in the lymphocyte gate were used for analysis. |
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