| The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.Applications Reported: The MB19-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1. Mucosal immunology A critical role for cellular inhibitor of protein 2 (cIAP2) in colitis-associated colorectal cancer and intestinal homeostasis mediated by the inflammasome and survival pathways."12-0191 was used in Immunofluorescence-paraffin to investigate the function of cellular inhibitors of apoptosis proteins in intestinal cancer and mechanisms in disease pathogenesis." Authors Dagenais M,Dupaul-Chicoine J,Champagne C,Skeldon A,Morizot A,Saleh M2. European journal of immunology Expression of a single ICAM-1 isoform on T cells is sufficient for development of experimental autoimmune encephalomyelitis."12-0191 was used in Flow cytometry/Cell sorting to assess the role of a particular ICAM-1 isoform expressed by T-cells in the development of of experimental autoimmune encephalomyelitis." Authors Bullard DC,Hu X,Crawford D,McDonald K,Ramos TN,Barnum SR3. Oncogene Minor cell-death defects but reduced tumor latency in mice lacking the BH3-only proteins Bad and Bmf. "12-0191 was used in Flow cytometry/Cell sorting to show that Bad and Bmf co-regulate lymphocyte homeostasis and limit spontaneous transformation in mice." Authors Baumgartner F,Woess C,Pedit V,Tzankov A,Labi V,Villunger A4. PloS one Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells. "12-0191 was used in Flow cytometry/Cell sorting to investigate the effect of Schistosoma mansoni infection on allergic airway inflammation, showing it was inhibited by IL-10 produced by CD1d(hi) B cells." Authors van der Vlugt LE,Labuda LA,Ozir-Fazalalikhan A,Lievers E,Gloudemans AK,Liu KY,Barr TA,Sparwasser T,Boon L,Ngoa UA,Feugap EN,Adegnika AA,Kremsner PG,Gray D,Yazdanbakhsh M,Smits HH5. PloS one Low CD4/CD8 T-cell ratio associated with inflammatory arthropathy in human T-cell leukemia virus type I Tax transgenic mice. "12-0191 was used in Flow cytometry/Cell sorting to identify the primary T-cell subsets involved in the development of arthropathy in Tax transgenic mice." Authors Ohsugi T,Kumasaka T |
QQ:1749072012