anti-mouse CD19 (eBio1D3) eFluor 506
货号：69-0193-82,69-0193-80
规格：100 μg,25 μg
价格：2090,1172
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：eBio1D3 (1D3)
荧光染料：eFluor 506
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow；MISC；FN |
| 推荐用法： | 0.5 µg/test（flow) |
| The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD19 Antibody (69-0193-82) in Flow Staining of BALB/c splenocytes withAnti-Mouse CD3e APC (Product # 17-0031-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 506 (Product # 69-4321-82) (left) or 0.25 µg of Anti-Mouse CD19 eFluor® 506 (right). Cells in the lymphocyte gate were used for analysis. |
| 推荐产品： |
| Flow Cytometry Staining Buffer, eBioscienceUltracomp eBeads microspheresLIVE/DEAD® Fixable Cell Stains |
| 参考文献： |
| 1.Journal of immunology (Baltimore, Md. : 1950)The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment.Authors Shoham T,Rajapaksa R,Boucheix C,Rubinstein E,Poe JC,Tedder TF,Levy S2.Blood Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement.Authors Inabe K,Kurosaki T3.Journal of immunology (Baltimore, Md. : 1950)The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens.Authors Cherukuri A,Cheng PC,Pierce SK |
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