| Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1. PloS one lkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity. 15-0193 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics." uthors Jonas BA,Johnson C,Gratzinger D,Majeti R2. Journal of immunology research rolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking. "15-0193 was used in Flow cytometry/Cell sorting to determine the effect of prolactin in an in vitro system of B-cell tolerance, showing that it rescues immature B-cells from apoptosis induced by B-cell receptor cross-linking." Authors Flores-Fernández R,Blanco-Favela F,Fuentes-Pananá EM,Chávez-Sánchez L,Gorocica-Rosete P,Pizaña-Venegas A,Chávez-Rueda AK3. Aging Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells. "15-0193 was used in Flow cytometry/Cell sorting to investigate the role of p16(Ink4a)/β-gal(pH6)-positive macrophages in aging, which previously was attributed solely to senescent cells." Authors Hall BM,Balan V,Gleiberman AS,Strom E,Krasnov P,Virtuoso LP,Rydkina E,Vujcic S,Balan K,Gitlin I,Leonova K,Polinsky A,Chernova OB,Gudkov AV4. Journal of immunology (Baltimore, Md. : 1950) Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice. "15-0193 was used in Flow cytometry/Cell sorting to demonstrate that chronic filarial infection suppresses eosinophilic responses to antibody-mediated activation." Authors Evans H,Killoran KE,Mitre BK,Morris CP,Kim SY,Mitre E5.mBio Toxoplasma gondii superinfection and virulence during secondary infection correlate with the exact ROP5/ROP18 allelic combination. "15-0193 was used in Flow cytometry/Cell sorting to investigate the interaction between infected host and Toxoplasma gondii strains in endemic regions, showing that secondary infection correlates with the ROP5/ROP18 allele combinations." Authors Jensen KD,Camejo A,Melo MB,Cordeiro C,Julien L,Grotenbreg GM,Frickel EM,Ploegh HL,Young L,Saeij JP |
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