| The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.应用报道: 抗体 eBio1D3 (1D3) 已报道可用于流式检测.应用验证: 抗体eBio1D3 (1D3) 已在小鼠脾细胞流式分析中应用验证. This can be used at less than or equal to 0.25 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered |
| 1. Cell reports Distinct Kinase-Independent Role of RIPK3 in CD11c+ Mononuclear Phagocytes in Cytokine-Induced Tissue Repair. "25-0193 was used in Flow cytometry/Cell sorting to study the distinct kinase-independent role of RIPK3 in cytokine-induced tissue repair." Authors Moriwaki K,Balaji S,Bertin J,Gough PJ,Chan FK2. The Journal of clinical investigation A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens. "25-0193 was used in Flow cytometry/Cell sorting to investigate the effect of Pr20 on cancer." Authors Chang AY,Dao T,Gejman RS,Jarvis CA,Scott A,Dubrovsky L,Mathias MD,Korontsvit T,Zakhaleva V,Curcio M,Hendrickson RC,Liu C,Scheinberg DA3. Nature communications Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. "25-0193 was used in Flow cytometry/Cell sorting to reveal a link between inflammation, IL-33 release by FALC stromal cells, ILC2 activation and pleural B-cell activation in FALCs, resulting in local and antigen-specific IgM production." Authors Jackson-Jones LH,Duncan SM,Magalhaes MS,Campbell SM,Maizels RM,McSorley HJ,Allen JE,Bénézech C4. Arthritis and rheumatology (Hoboken, N.J.) Development of Th17-Associated Interstitial Kidney Inflammation in Lupus-Prone Mice Lacking the Gene Encoding STAT-1. "25-0193 was used in Flow cytometry/Cell sorting to investigate the roles of the type I and type II IFN signaling components in systemic Lupus Erythematosus, showing the development of Th17-associated interstitial kidney inflammation in mice lacking STAT-1." Authors Yiu G,Rasmussen TK,Ajami B,Haddon DJ,Chu AD,Tangsombatvisit S,Haynes WA,Diep V,Steinman L,Faix J,Utz PJ5.Pflugers Archiv : European journal of physiology The impact of chronic intermittent hypoxia on hematopoiesis and the bone marrow microenvironment. "25-0193 was used in Flow cytometry/Cell sorting to investigate how chronic intermittent hypoxia affects hematopoiesis and the bone marrow microenvironment." Authors Alvarez-Martins I,Remédio L,Matias I,Diogo LN,Monteiro EC,Dias S |
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