Anti-mouse CD19 （eBio1D3）SB436
货号：62-0193-82,62-0193-80
规格：100 μg,25 μg
价格：3096,0
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：eBio1D3 (1D3)
荧光染料：Super Bright 436
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow |
| 推荐用法： | 0.5 µg/test（flow) |
| The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser |
| 数据 |
CD19 Antibody (62-0193-82) in Flow C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0031) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321) (left) or 0.25 µg of CD19 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis. |
| 推荐产品： |
| Super Bright Staining Buffer, eBioscienceUltracomp eBeads microspheresLIVE/DEAD® Fixable Cell Stains |
| 参考文献： |
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