Anti-mouse CD19 (eBio1D3) Alexa Fluor700
货号：56-0193-80,56-0193-82
规格：25μg,100μg
价格：1180,2388
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：eBio1D3 (1D3)
荧光染料：Alexa Fluor 700
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow；FN |
| 推荐用法： | 0.25 µg/test（flow) |
| Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control Alexa Fluor® 700 (Product # 56-4321-80) (left) or 0.125 µg of Anti-Mouse CD19 Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Cell reportsVaccination with Necroptic Efficiet AntitumorImmunity"56-0193was usedin Flowcytomety/Cellsorting toshowthatnecroptticcancercellsreleasedamageassociaedmoleculrpatternsandpromotematuraton of dendritic cells, the cross-priming of cytotoxic T cells, and the production of IFN-γ."Authors Aaes TL,Kaczmarek A,Delvaeye T,De Craene B,De Koker S,Heyndrickx L,Delrue I,Taminau J,Wiernicki B,De Groote P,Garg AD,Leybaert L,Grooten J,Bertrand MJ,Agostinis P,Berx2.Journal of neuroinflammationProtection from experimental autoimmune encephalomyelitis by polyclonal IgG requires adjuvant-induced inflammation."56-0193 was used in Flow cytometry/Cell sorting to identify factors affecting the efficacy of intravenous immunoglobulin treatment for multiple sclerosis." |
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