| Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1. Cell stem cellAdipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration."47-0193 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone."Authors Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ2.Scientific reportsp21Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARα."47-0193 was used in Flow cytometry/Cell sorting to examine the expression of several tumour suppressors upon fasting in mice."Authors Lopez-Guadamillas E,Fernandez-Marcos PJ,Pantoja C,Muñoz-Martin M,Martínez D,Gómez-López G,Campos-Olivas R,Valverde AM,Serrano M3.Infection and immunityImpaired innate immunity in mice deficient in interleukin-1 receptor-associated kinase 4 leads to defective type 1 T cell responses, B cell expansion, and enhanced susceptibility to infection with Toxoplasma gondii."47-0193 was used in Flow cytometry/Cell sorting to investigate the role of IRAK4 in Toxoplasma gondii infection, showing that deficiency leads to impaired innate immunity."Authors Béla SR,Dutra MS,Mui E,Montpetit A,Oliveira FS,Oliveira SC,Arantes RM,Antonelli LR,McLeod R,Gazzinelli RT4.Journal of immunology (Baltimore, Md. : 1950)The signaling activity of murine CD19 is regulated during cell development.Authors Krop I,Shaffer AL,Fearon DT,Schlissel MS5.European journal of immunologySelf-renewal of B-1 lymphocytes is dependent on CD19.Authors Krop I,de Fougerolles AR,Hardy RR,Allison M,Schlissel MS,Fearon DT |
QQ:1749072012