eBioscience™ Phytohemagglutinin-L (PHA-L) Solution (500X)/PHA广谱激活剂

2024-10-16

eBioscience™ Phytohemagglutinin-L (PHA-L) Solution (500X)/PHA广谱激活剂

货号:00-4977-03,00-4977-93

规格:4 x 100 µL,100 µL

价格:2110,786

产品类型:免疫细胞培养

品牌:eBioscience

Phytohemagglutinin-L (PHA-L) Solution (500X) 是一款即用型 PHA-L 缓冲水溶液。PHA 是从红芸豆即菜豆中分离的凝集素混合物。PHA-L 是 PHA 的一部分,能产生强烈的白血球凝集反应并具有促有丝分裂活性,但是几乎没有红细胞凝集反应活性。这种试剂设计用于人类和小鼠白细胞的体外活化。
数据:

图1 Data for Phytohemagglutinin-L (PHA-L) Solution (500X)Left: Human peripheral blood mononuclear cells were labeled with 10 µM Cell Proliferation Dye eFluor™ 450 (cat. 65-0842) and cultured for 3 days with Phytohemagglutinin-L (PHA-L) Solution (500X) at 2 uL per mL of culture medium. Cells were stained with Anti-Human CD86 PE (cat. 12-0869) and Fixable Viability Dye eFluor™ 660 (cat. 65-0864). Total singlet-gated, viable cells were used for analysis.Right: Mouse splenocytes were labeled with 10 µM Cell Proliferation Dye eFluor™ 450 (cat. 65-0842) and cultured for 3 days with Phytohemagglutinin-L (PHA-L) Solution (500X) at 2 uL per mL of culture medium. Cells were stained with Anti-Mouse CD86 PE-Cyanine7 (cat. 25-0862) and Fixable Viability Dye eFluor™ 660 (cat. 65-0864). Total singlet-gated, viable cells were used for analysis.
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技术参数
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