CD19 Monoclonal Antibody (SJ25C1), PE-Cyanine5.5
货号：35-0198-42
规格：100TESTS
价格：3107
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：SJ25C1
荧光染料：PE-Cy5.5
| 抗体类型: | 单抗 | 同型对照： | IGM |
| 免疫原性： | Human embryonal carcinoma (EC) cell line 2102Ep | 用法： | The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. |
| Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-567 nm; Emission: 690 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. |
| 数据 |
CD19 Antibody (35-0198-42) in Flow Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 K Isotype Control PE-Cyanine5-5 (Product # 35-4714-82) (left) or Anti-Human CD19 PE-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis. |
| 推荐产品： |
| ANTI-HUMAN CD19 (HIB19) FITC（货号#11-0199）ANTI-HUMAN CD19 (SJ25C1) PE（货号#12-0198）ANTI-HUMAN CD19 (HIB19) PE（货号#12-0199）ANTI-HUMAN CD19 (HIB19) PE (货号#12-0199-80） |
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