SARS-CoV-2 Spike Protein (RBD) Recombinant Human Monoclonal Antibody (P05DHu), Alexa Fluor 488
货号：53-6490-82
规格：100 µg
价格：0
产品类型：流式抗体
品牌：eBioscience
物种：其它
宿主：其它
抗体亚型：IgG1, kappa
克隆号：P05DHu
荧光染料：Alexa Fluor 488
| 抗体类型 | 单抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 1 μg/test (Flow) |
| 产品详细信息Description: This antibody recognizes the RBD domain of SARS CoV2 Spike protein.Applications Tested: This P05DHu Alexa Fluor™ 488 conjugate has been tested by flow cytometric analysis of HEK 293 cells transfected with a vector encoding full length spike protein from SARS CoV2. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically butcan range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue LaserFiltration: 0.2 µm post-manufacturing filtered.靶标信息SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. |
| 数据 |
| SARS-CoV-2 Spike Protein (RBD) Antibody (53-6490-82) in FlowHEK 293 cells were transfected with a vector encoding full length spike protein from SARS CoV2 (pink histogram) or left untransfected (blue histogram) and then stained with 1 µg of Anti-SARS CoV2 Spike Protein RBD Recombinant Human Monoclonal Antibody, Alexa Fluor 488 (Product # 53-6490-82). Viable cells were used for analysis as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18). |
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