CaspGLOW™ Fluorescein Active Caspase Staining Kit
货号:88-7003-42
规格:100 tests
价格:3942
产品类型:细胞凋亡检测
品牌:Thermo Fisher
物种:恒河猴/小鼠/大鼠
宿主:小鼠
抗体亚型:IgG
荧光染料:eFluor 506
CaspGLOW™ Fluorescein Active Staining Kit 含有能灵敏检测哺乳动物细胞中活性 Caspase 所需的试剂。该测定利用专门针对所有 Caspase 的抑制剂 Z-VAD-FMK 直接偶联至 FITC(异硫氰酸荧光素)作为检测系统。该试剂能够透过细胞、无毒,并且不可逆地结合上活性酶。通过流式细胞术、荧光显微术或者荧光板读数仪可检测标记细胞。Caspase (半胱天冬酶) 是一系列天冬氨酸特异性半胱氨酸蛋白酶,如激酶一样可以进行逐步信号转导反应。这些蛋白酶募集形成低聚受体可致其激活并伴随自我蛋白酶解。有活性的半胱天冬酶可以蛋白水解其他半光天冬酶产生级联反应。这种信号通路最终结果是程序性细胞死亡,称为细胞凋亡。 |
数据: |
图1.Data for CaspGLOW™ Fluorescein Active Caspase Staining KitMouse thymocytes were cultured overnight in complete medium to induce apoptosis, followed by a 30 minute incubation using the CaspGLOW™ Fluorescein Active Caspase Staining Kit and protocol. Cells were then harvested, washed and stained with Annexin V Apoptosis Detection Kit APC (cat. 88-8007). Finally, the cells were analyzed in the presence of 7-AAD Viability Staining Solution (cat. 00-6993). Viable cells, as determined by exclusion of 7-AAD (left), were analyzed for staining of Annexin V and CaspGLOW™ Fluorescein Active Caspase (right). |
相关产品: |
▪细胞周期检测▪细胞凋亡检测▪细胞增殖活性检测 |
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