Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.2016

"17-0037 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which cytokines in the blood affect HSC homeostasis, showing that LECT2 drives HSC expansion and mobilisation."

Authors Lu XJ,Chen Q,Rong YJ,Yang GJ,Li CH,Xu NY,Yu CH,Wang HY,Zhang S,Shi YH,Chen J

Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.2016

"17-0037 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses."

Authors Tomić A,Varanasi PR,Golemac M,Malić S,Riese P,Borst EM,Mischak-Weissinger E,Guzmán CA,Krmpotić A,Jonjić S,Messerle M

Differences of IL-1β Receptors Expression by Immunocompetent Cells Subsets in Rheumatoid Arthritis.2016

"17-0037 was used in Flow cytometry/Cell sorting to study immune cell types and their differential expression of receptors to immunomodulatory cytokines in healthy and arthritic individuals."

Authors Alshevskaya AA,Lopatnikova JA,Shkaruba NS,Chumasova OA,Sizikov AE,Karaulov AV,Kozlov VA,Sennikov SV

Peanut oral immunotherapy transiently expands circulating Ara h 2-specific B cells with a homologous repertoire in unrelated subjects.2015

"17-0037 was used in Flow cytometry/Cell sorting to investigate the contribution of induced antibody clones to clinical tolerance in peanut oral immunotherapy (PNOIT), showing that PNOIT transiently expands circulating Ara h 2-specific B cells."

Authors Patil SU,Ogunniyi AO,Calatroni A,Tadigotla VR,Ruiter B,Ma A,Moon J,Love JC,Shreffler WG

Peanut oral immunotherapy transiently expands circulating Ara h 2-specific B cells with a homologous repertoire in unrelated subjects.2015

"17-0037 was used in Flow cytometry/Cell sorting to investigate the contribution of induced antibody clones to clinical tolerance in peanut oral immunotherapy (PNOIT), showing that PNOIT transiently expands circulating Ara h 2-specific B cells."

Authors Patil SU,Ogunniyi AO,Calatroni A,Tadigotla VR,Ruiter B,Ma A,Moon J,Love JC,Shreffler WG

Relationship between interleukin-1 type 1 and 2 receptor gene polymorphisms and the expression level of membrane-bound receptors.

"17-0037 was used in Flow cytometry/Cell sorting to determine the relationship between interleukin-1 type 1 and type 2 receptor gene polymorphisms and the expression level of membrane-bound IL1-Rs."

Authors Vasilyev FF,Silkov AN,Sennikov SV