CD3 Monoclonal Antibody (OKT3), Alexa Fluor 700, eBioscience™-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

CD3 Monoclonal Antibody (OKT3), Alexa Fluor 700, eBioscience™

2024-10-16

CD3 Monoclonal Antibody (OKT3), Alexa Fluor 700, eBioscience™

货号：56-0037-42

规格：100TESTS

价格：3107

产品类型：流式抗体

品牌：eBioscience

抗原：CD3

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：OKT3

荧光染料：Alexa Fluor 700

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	5uL（0.5 µg）/test(Flow）

Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.

数据

CD3 Antibody (56-0037-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG2a K Isotype Control Alexa Fluor® 700 (Product # 56-4724-80) (left) or Anti-Human CD3 Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.JCI insight

B cells from African American lupus patients exhibit an activated phenotype.

"56-0037 was used in Flow cytometry/Cell sorting to investigate immunological differences between African American and European American patients with systemic lupus erythematosus, showing that B cells from African American patients exhibit an activated phenotype."

Authors Menard LC,Habte S,Gonsiorek W,Lee D,Banas D,Holloway DA,Manjarrez-Orduno N,Cunningham M,Stetsko D,Casano F,Kansal S,Davis PM,Carman J,Zhang CK,Abidi F,Furie R,Nadler SG,Suchard SJ

2.PLoS pathogens

Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

"56-0037 was used in Flow cytometry/Cell sorting to further define the immunological footprint of Mycobacterium tuberculosis CD4 T cell recognition."

Authors Lindestam Arlehamn CS,Gerasimova A,Mele F,Henderson R,Swann J,Greenbaum JA,Kim Y,Sidney J,James EA,Taplitz R,McKinney DM,Kwok WW,Grey H,Sallusto F,Peters B,Sette A

技术参数

产品应用 Flow

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CD3 Monoclonal Antibody (OKT3), Alexa Fluor 700, eBioscience™

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