| Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.BloodDistinct signaling programs control human hematopoietic stem cell survival and proliferation.2015"12-0909 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms by which distinct signalling programs control human hematopoietic stem cell survival and proliferation."Authors Knapp DJ,Hammond CA,Aghaeepour N,Miller PH,Pellacani D,Beer PA,Sachs K,Qiao W,Wang W,Humphries RK,Sauvageau G,Zandstra PW,Bendall SC,Nolan GP,Hansen C,Eaves CJ2.Stem cell research and therapyVitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells.2016"12-0909 was used in Flow cytometry/Cell sorting to evaluate whether porcine adipose-derived MSCs can metabolise and respond to vitamin D."Authors Valle YL,Almalki SG,Agrawal DK3.Stem cell researchImpaired function of bone marrow stromal cells in systemic mastocytosis.2015"12-0909 was used in Flow cytometry/Cell sorting to implicate epigenetic change in mesenchymal stem cells as a driver of some of the symptoms associated with systemic mastocytosis."Authors Nemeth K,Wilson TM,Ren JJ,Sabatino M,Stroncek DM,Krepuska M,Bai Y,Robey PG,Metcalfe DD,Mezey E |
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