ANTI-HUMAN CD90 (EBIO5E10) BIOTIN
货号:13-0909-80,13-0909-82
规格:25ug,100ug
价格:0,2271
产品类型:流式抗体
品牌:eBioscience
抗原:CD90
物种:人
宿主:小鼠
抗体亚型:IgG
克隆号:EBIO5E10
荧光染料:Biotin
抗体类型: | 单抗 | 同型对照: | IgG1, kappa |
免疫原性: | | 用法: | 1 µg/test(Flow) |
Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio5E10 (5E10) antibody has been tested by flow cytometrric analysis of human erythroleukemia (HEL) cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered. |
数据 |
CD90 (Thy-1) Antibody (13-0909-80) in FlowStaining of HEL cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.5 µg of Anti-Human CD90 (Thy-1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. |
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参考文献: |
1.The Journal of experimental medicineDistinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory.2012"13-0909 was used in Flow cytometry/Cell sorting to reveal distinct cellular pathways that select germline-encoded and somatically mutated antibodies into immunological memory."Authors Kaji T,Ishige A,Hikida M,Taka J,Hijikata A,Kubo M,Nagashima T,Takahashi Y,Kurosaki T,Okada M,Ohara O,Rajewsky K,Takemori T |
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