| Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.Applications Tested: This eBio5E10 (5E10) antibody has been tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.Journal of translational medicineEndothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.2017"14090982 was used in immunocytochemistry to examine if endothelial and smooth muscle cells acquired from post-surgically discarded cardiac tissue can be used in cell replacement therapy"Authors Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM2.Journal of applied physiology (Bethesda, Md. : 1985)Pericyte response to contraction mode-specific resistance exercise training in human skeletal muscle.2015"14090982 was used in immunohistochemistry to report that resistance training leads to a decline in pericyte quantity and to an increase in mesenchymal progenitor cell proliferation"Authors Farup J,De Lisio M,Rahbek SK,Bjerre J,Vendelbo MH,Boppart MD,Vissing K3.AIDS (London, England)Role of intestinal myofibroblasts in HIV-associated intestinal collagen deposition and immune reconstitution following combination antiretroviral therapy.2015"14-0909 was used in Immunohistochemistry on frozen tissues to investigate the potential role of mucosal intestinal myofibroblasts in HIV and associated fibrosis in gut-associated lymphoid tissue."Authors Asmuth DM,Pinchuk IV,Wu J,Vargas G,Chen X,Mann S,Albanese A,Ma ZM,Saroufeem R,Melcher GP,Troia-Cancio P,Torok NJ,Miller CJ,Powell DW4.Nucleic acids researchMulti-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.2015"14090982 was used in flow cytometry to describe methods to efficiently 'knock-in' multi-kilobase genes in human induced pluripotent stem cells"Authors Byrne SM,Ortiz L,Mali P,Aach J,Church GM5.Nucleic acids researchMulti-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.1993"14090982 was used in flow cytometry to describe methods to efficiently 'knock-in' multi-kilobase genes in human induced pluripotent stem cells"Authors Byrne SM,Ortiz L,Mali P,Aach J,Church GM |
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