ANTI-HUMAN CD90 (EBIO5E10) PE-CY7
货号：25-0909-41,25-0909-42
规格：25T,100T
价格：1474,3679
产品类型：流式抗体
品牌：eBioscience
抗原：CD90
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：EBIO5E10
荧光染料：PE-Cy7
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（0.25µg）/test(Flow） |
| Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kDa receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD90 (Thy-1) Antibody (25-0909-41) in FlowStaining of human erythroleukemia (HEL) cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD90 (Thy-1) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis. |
| 推荐产品： |
| 间充干细胞MSC分选间充干细胞MSC扩增、分化培养基CD90流式鉴定细胞因子 |
| 参考文献： |
| 1.Proceedings of the National Academy of Sciences of the United States of AmericaDevelopment of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus.2015"25-0909 was used in Flow cytometry/Cell sorting to study T-cell development in the thymus at a clonal level, with an aim to improve immune reconstitution following hematopoietic stem cell transplant."Authors Brugman MH,Wiekmeijer AS,van Eggermond M,Wolvers-Tettero I,Langerak AW,de Haas EF,Bystrykh LV,van Rood JJ,de Haan G,Fibbe WE,Staal FJ2.Journal of autoimmunityImmunopathogenic role of TH1 cells in autoimmune diabetes: evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model.2005Authors Hung JT,Liao JH,Lin YC,Chang HY,Wu SF,Chang TH,Kung JT,Hsieh SL,McDevitt H,Sytwu HK3.BloodThy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood.1994Authors Mayani H,Lansdorp PM4.The Journal of experimental medicineExpression of Thy-1 on human hematopoietic progenitor cells.1993Authors Craig W,Kay R,Cutler RL,Lansdorp PM |
技术参数 产品应用 Flow；IP；WB；MISC；IHC
细胞类型 MSC
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