CD73 Monoclonal Antibody (AD2), FITC

货号：11-0739-41,11-0739-42

规格：25Tests,100Tests

价格：1060,2117

产品类型：流式抗体

品牌：eBioscience

抗原：CD73

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：AD2

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC,
浓度：	5 µL/Test	用法：	5uL（0.25 µg）/test(Flow）

产品详细信息Description: This AD2 monoclonal antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.Applications Reported: This AD2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AD2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.

数据

CD73 Antibody (11-0739-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD73 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Stem cell reportsMIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.2016"11-0739 was used in Flow cytometry/Cell sorting to show MIF plays a role in regulating the differentiation fate of human cartilage endplate stem cells under hypoxia."Authors Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y2.Scientific reportsPotency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing.2016"11-0739 was used in Flow cytometry/Cell sorting to determine that umbilical cord blood offers a potential source of MSCs for therapeutic scarless wound healing."Authors Doi H,Kitajima Y,Luo L,Yan C,Tateishi S,Ono Y,Urata Y,Goto S,Mori R,Masuzaki H,Shimokawa I,Hirano A,Li TS3.Scientific reportsFocal Adhesion Kinase Signaling Mediated the Enhancement of Osteogenesis of Human Mesenchymal Stem Cells Induced by Extracorporeal Shockwave.2016"11-0739 was used in Flow cytometry/Cell sorting to investigate the underlying mechanisms of osteogenic effects of bone marrow mesenchymal stem cells induced by extracorporeal shockwave."Authors Hu J,Liao H,Ma Z,Chen H,Huang Z,Zhang Y,Yu M,Chen Y,Xu J4.Stem cells translational medicineComparison of human adipose-derived stem cells isolated from subcutaneous, omental, and intrathoracic adipose tissue depots for regenerative applications.2014"11-0739 was used in Flow cytometry/Cell sorting to determine that depot selection is an important factor to consider when applying ASCs in tissue-specific cell-based regenerative therapies."Authors Russo V,Yu C,Belliveau P,Hamilton A,Flynn LE5.OncotargetTumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.2014"11-0739 was used in Flow cytometry/Cell sorting to show that tumour-derived exosomes promote tumour progression and T-cell dysfunction through regulation of exosomal microRNAs in human carcinomas."Authors Ye SB,Li ZL,Luo DH,Huang BJ,Chen YS,Zhang XS,Cui J,Zeng YX,Li J

技术参数

产品应用 Flow

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