ANTI-HUMAN CD73 (AD2) APC

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ANTI-HUMAN CD73 (AD2) APC

货号:17-0739-41,17-0739-42

规格:25T,100T

价格:1411,3298

产品类型:流式抗体

品牌:eBioscience

抗原:CD73

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:AD2

荧光染料:APC

抗体类型:单抗同型对照:IgG1, kappa
免疫原性:用法:5uL(0.125 µg)/test(Flow)
Description: This AD2 monoclonal antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.Applications Reported: This AD2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AD2 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD73 Antibody (17-0739-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD73 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.
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参考文献:
1.PloS oneSpontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.2016"17-0739 was used in Flow cytometry/Cell sorting to investigate the combination of MSCs and NFs as a therapeutic technique for the repair of joints affected by bone and cartilage destruction."Authors Sonomoto K,Yamaoka K,Kaneko H,Yamagata K,Sakata K,Zhang X,Kondo M,Zenke Y,Sabanai K,Nakayamada S,Sakai A,Tanaka Y2.OncotargetFOXF1 mediates mesenchymal stem cell fusion-induced reprogramming of lung cancer cells.2014"17-0739 was used in Flow cytometry/Cell sorting to study the effect of fusion of mesenchymal stem cells with lung cancer cells, showing that fusion is mediated by FOXF1 and leads to a more benign state due to complementation of tumorigenic defects such as the restoration of p21 function."Authors Wei HJ,Nickoloff JA,Chen WH,Liu HY,Lo WC,Chang YT,Yang PC,Wu CW,Williams DF,Gelovani JG,Deng WP3.Journal of immunology (Baltimore, Md. : 1950)Characterization of an IgM Fc-binding receptor on human T cells.1993Authors Nakamura T,Kubagawa H,Ohno T,Cooper MD

技术参数

产品应用 Flow;MISC

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