ANTI-HUMAN CD45 (CD45-2B11) PURIFIED
货号：14-9457-82,14-9457-80,14-9457-37,14-9457-95
规格：100ug,25ug,2 mg,10 mg
价格：1304,0,11920,29802
产品类型：流式抗体
品牌：eBioscience
抗原：CD45
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：CD45-2B11
荧光染料：其它
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 0.25 µg/test（flow）；10 µg/mL（IHC(p)）;5 µg/mL（WB） |
| Description: The CD45-2B11 monoclonal antibody reacts with human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in lymphocyte proliferation and differentiation. The 2B11 antibody is useful for recognition of normal and neoplastic lymphoid cells.Applications Reported: This CD45-2B11 antibody has been reported for use in flow cytometric analysis, immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This CD45-2B11 antibody has been tested by immunohistochemisty on formalin-fixed paraffin embedded tissue using high pH antigen retrieval. This can be used at less than or equal to 10 µg/mL. This CD45-2B11 antibody has also been tested by western blot at 5 µg/mL and by flow cytometric analysis on human peripheral blood cells at 0.25 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD45 Antibody (14-9457-82) in IHC (P)Immunohistochemistry on formalin-fixed paraffin embedded human pancreas, using 10 µg/mL of Mouse IgG1 K Isotype Control (left) or 10 µg/mL Anti-Human CD45 Purified (right) followed by Anti-Mouse IgG Biotin, and DAB visualization.Nuclei are counterstained with hematoxylin. |
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| 参考文献： |
| 1.JCI insightHeterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney.2016"14-9457 was used in Immunohistochemistry to show how the inhibition of tertiary lymphoid tissue may constitute a therapeutic approach for treating acute kidney injury in the elderly."Authors Sato Y,Mii A,Hamazaki Y,Fujita H,Nakata H,Masuda K,Nishiyama S,Shibuya S,Haga H,Ogawa O,Shimizu A,Narumiya S,Kaisho T,Arita M,Yanagisawa M,Miyasaka M,Sharma K,Minato N,Kawamoto H,Yanagita M2.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, IncInflammatory malignant fibrous histiocytoma: distinction from Hodgkin's disease and non-Hodgkin's lymphoma by a panel of leukocyte markers.1997Authors Khalidi HS,Singleton TP,Weiss SW |
技术参数 产品应用 Flow；ICC；IF；IHC（P）；WB
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