DyLight 650 Maleimide/DyLight 650马来酰亚胺
货号：62295
规格：1 mg
价格：4327
产品类型：蛋白标记
品牌：Thermo Fisher
| Thermo Scientific DyLight 650 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 650 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.DyLight 650provides vibrant far-red fluorescence with comparable or improved performance over other dyes, including Alexa Fluor 647 and Cy5 dye, over a broad pH range (pH 4-9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.yLight 650 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more aboutmaleimide chemistry. |
| 特点 |
| •High performance—DyLight 650 fluoresces brighter than Alexa Fluor 647 and Cy5•Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)•Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling•Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency |
| 应用 |
| • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay• Target macromolecule labeling forin vitroandin vivofluorescent detection strategies |
| 数据 |
Immunofluorescence microscopy using DyLight 650 dyeIntermediate filaments (red) in A549 cells were fluorescently labeled with biotinylated mouse anti-cytokeratin 18 antibody and DyLight 650-Conjugated Streptavidin. Nuclei were labeled with Hoechst dye. |
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