ANTI-HUMAN CD45 (HI30) PE-CY5
货号：15-0459-42
规格：100T
价格：2713
产品类型：细胞生物学
品牌：eBioscience
| 抗体类型: | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5 |
| 免疫原性： | | 用法： | 5uL（0.015µg）/test(Flow） |
| Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD45 Antibody (15-0459-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD45 PE-Cyanine5 (filled histogram).Total viable cells were used for analysis. |
| 推荐产品： |
| 间充干细胞MSC分选间充干细胞MSC扩增、分化培养基CD45流式抗体细胞因子 |
| 参考文献： |
| 1.The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung DiseaseA new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.2008"15045942 was used in flow cytometry to demonstrate that aerosolized interferon-gamma results in the transient conversion of sputum smears in multidrug-resistant pulmonary tuberculosis"Authors Grahmann PR,Braun RK |
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